Techniques of molecular biology
On behalf of the Journal of Biology and Medical-Research, as Editor-in-Chief, it is my distinct honour and privilege to inform you that, it’s been years we have started the Journal, now we are celebrating the Anniversary and we are privileged to welcome Analytical Society to our journal. As Editor-In-Chief it is my great pleasure and honour to welcome you to our Journal of Biology and Medical-Research.
The Journal of Biology and Medical-Research aims to disseminate knowledge and promote discussion through the publication of peer-reviewed, high quality research papers on all topics related to like molecular biology, theoretical biology, structural biology, marine biology etc. The open access journal is published by Insight Medical Publishing (iMedPub) who hosts open access peer-reviewed journals as well as organizes more than 100 International scientific Conferences.
Techniques of molecular biology
One of the most basic techniques of molecular biology to study protein function is molecular cloning. In this technique, DNA coding for a protein of interest is cloned using polymerase chain reaction (PCR), and/or restriction enzymes into a plasmid (expression vector). A vector has 3 distinctive features: an origin of replication, a multiple cloning site (MCS), and a selective marker usually antibiotic resistance. Located upstream of the multiple cloning site are the promoter regions and the transcription start site which regulate the expression of cloned gene. This plasmid can be inserted into either bacterial or animal cells.
Introducing DNA into bacterial cells can be done by transformation via uptake of naked DNA, conjugation via cell-cell contact or by transduction via viral vector. Introducing DNA into eukaryotic cells, such as animal cells, by physical or chemical means is called transfection. Several different transfection techniques are available, such as calcium phosphate transfection, electroporation, microinjection and liposome transfection. The plasmid may be integrated into the genome, resulting in a stable transfection, or may remain independent of the genome, called transient transfection.
DNA coding for a protein of interest is now inside a cell, and the protein can now be expressed. A variety of systems, such as inducible promoters and specific cell-signaling factors, are available to help express the protein of interest at high levels. Large quantities of a protein can then be extracted from the bacterial or eukaryotic cell.
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